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biotinylated secondary antibody for peroxidase  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated secondary antibody for peroxidase
    Biotinylated Secondary Antibody For Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectastain+abc+hrp+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Mouse+IgG)/pm42073608-108-5-12
    Average 96 stars, based on 1140 article reviews
    biotinylated secondary antibody for peroxidase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Long-Term Hyperglycemia Affects the Expression of Diaph1 and Its Cytoskeleton Ligands in the Epidermis of Diabetic Patients—A Quantitative Study
    Article Snippet: For immunofluorescence, sections were rinsed in PBS and incubated with secondary goat anti-rabbit antibodies, i.e., Alexa 594 or Alexa 488 (A-11037 or A-11034, 1:1000, Invitrogen, Thermo Fisher, Waltham, MA, USA) for one hour at room temperature, rinsed in PBS and cover-slipped with DAPI (4′,6-Diamidino-2-phenylindole) mounting solution (Thermo Fisher, Waltham, MA, USA). .. The ABC-HRP (Avidin-Biotin ComplexHorseradish Peroxidase) kit protocol was followed (Vectastain ABC-HRP kit, Vector Laboratories, Newark, CA, USA) using DAB (3,3′-Diaminobenzidine) as chromogen. ..

    Article Title: Audiogenic Kindling Stimulation Fails to Induce Cerebral Overexpression of P-Glycoprotein and Limbic Crises in the GASH/Sal Model of Epilepsy
    Article Snippet: After, sections were washed and incubated for 2 h at room temperature with a biotinylated goat anti-rabbit secondary antibody (1/200, Vector Laboratories, Newark, CA, USA, Cat. No. BA-1000). .. Following secondary incubation, the avidin–biotin complex (ABC) complex was applied for 90 min (VECTASTAIN® ABC-HRP Kit, Vector Laboratories, Cat. No. PK-4000). .. Immunolabeling was visualized using 3,3-diaminobenzidine tetrahydrochloride (DAB) chromogen (ImmPACT DAB Peroxidase Substrate, Vector Laboratories, Cat. No. SK-4105) under visual and microscopic control.

    Incubation:

    Article Title: Pathogenic IgG from long COVID patients with neurological sequelae triggers sensitive but not cognitive impairments upon transfer into mice
    Article Snippet: .. Sections were then incubated with secondary antibodies (Vectastain ABC-HRP kit, Peroxidase [Mouse IgG] PK-4002 or [Rabbit IgG] PK-4001 or [Goat IgG] PK-4005, Vector Laboratories, Newark, CA, USA) for 30 min at RT. .. Immunolabeling was revealed using 3,3’-diaminobenzidine (DAB, K3468, Dako, Santa Clara, CA, USA).

    Article Title: Artemisinin attenuates 3-nitropropionic acid-induced neurodegeneration via HMGB1/TLR4/NF-κB modulation in a rat model of huntington's disease.
    Article Snippet: .. Following incubation with primary monoclonal antibodies, sections were treated with reagents from the Vectastain ABC-HRP kit (Vector Labs). .. Antigen–antibody binding was visualized by peroxidase-mediated diaminobenzidine (DAB; Sigma) staining.

    Article Title: Audiogenic Kindling Stimulation Fails to Induce Cerebral Overexpression of P-Glycoprotein and Limbic Crises in the GASH/Sal Model of Epilepsy
    Article Snippet: After, sections were washed and incubated for 2 h at room temperature with a biotinylated goat anti-rabbit secondary antibody (1/200, Vector Laboratories, Newark, CA, USA, Cat. No. BA-1000). .. Following secondary incubation, the avidin–biotin complex (ABC) complex was applied for 90 min (VECTASTAIN® ABC-HRP Kit, Vector Laboratories, Cat. No. PK-4000). .. Immunolabeling was visualized using 3,3-diaminobenzidine tetrahydrochloride (DAB) chromogen (ImmPACT DAB Peroxidase Substrate, Vector Laboratories, Cat. No. SK-4105) under visual and microscopic control.

    Article Title: Licofelone-Loaded Poly(d,l-Lactide-Co-Glycolide) Nanoparticles to Enhance Therapeutic Action: A Novel Approach for the Topical Management of Irritant Contact Dermatitis.
    Article Snippet: The sections were then incubated with primary antibodies specific to interleukin-1β (IL-1β; Cat. No. E-AB-33471, Elabscience, Wuhan, China) and tumor necrosis factor-α (TNF-α; Cat. No. GB11188, Servicebio, Wuhan, China). .. Following primary antibody incubation, the reagents required for signal amplification were applied according to the ABC method applying a Vectastain® ABC-HRP kit (Vector Laboratories, USA), in accordance with the manufacturer’s protocol. .. Immunohistochemical marker expression was visualized through peroxidase labeling, using 3,3′-diaminobenzidine (DAB; Sigma) as the chromogenic substrate to detect antigen–antibody complexes.

    Bioprocessing:

    Article Title: Artemisinin attenuates 3-nitropropionic acid-induced neurodegeneration via HMGB1/TLR4/NF-κB modulation in a rat model of huntington's disease.
    Article Snippet: .. Following incubation with primary monoclonal antibodies, sections were treated with reagents from the Vectastain ABC-HRP kit (Vector Labs). .. Antigen–antibody binding was visualized by peroxidase-mediated diaminobenzidine (DAB; Sigma) staining.

    Amplification:

    Article Title: Licofelone-Loaded Poly(d,l-Lactide-Co-Glycolide) Nanoparticles to Enhance Therapeutic Action: A Novel Approach for the Topical Management of Irritant Contact Dermatitis.
    Article Snippet: The sections were then incubated with primary antibodies specific to interleukin-1β (IL-1β; Cat. No. E-AB-33471, Elabscience, Wuhan, China) and tumor necrosis factor-α (TNF-α; Cat. No. GB11188, Servicebio, Wuhan, China). .. Following primary antibody incubation, the reagents required for signal amplification were applied according to the ABC method applying a Vectastain® ABC-HRP kit (Vector Laboratories, USA), in accordance with the manufacturer’s protocol. .. Immunohistochemical marker expression was visualized through peroxidase labeling, using 3,3′-diaminobenzidine (DAB; Sigma) as the chromogenic substrate to detect antigen–antibody complexes.



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